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Efficient Oil-Eating Bacteria Cooperate to Maximize Dining Capacity

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Researchers from the University of Tsukuba in collaboration with French researchers have discovered that oil-degrading bacteria, widely found in the ocean, grow around oil droplets forming dense communities at the oil-water interface. These communities cooperate to bend and buckle the oil interface, which enlarges its area and thereby allows more bacterial cells to come into direct contact with the oil for efficient oil degradation.

Tsukuba, Japan—Oil spills are disastrous for the natural environment, as they lead to long lasting pollution that harms surrounding wildlife. However, certain oil-degrading bacteria thrive in oil-spill conditions and contribute remarkably to the bioremediation of oil. Although biofilms, which are communities formed by bacteria, play a crucial role in the bioremediation of oil, how biofilms engage with oil in the ocean has remained unclear.

Researchers have made significant progress in clarifying the link between biofilm formation and oil degradation using a state-of-the-art microfluidic observation system. Combined with high-resolution confocal microscopes, this system helps to visualize the interactions between bacterial cells and microscopic oil droplets that measure about half the diameter of a hair. Their findings show that an oil-degrading bacterium Alcanivorax borkumensis (meaning "devourer of alkanes") forms biofilms that consume the oil by surrounding and adhering strongly to the oil-water interface. The cell community stretches the oil droplets into numerous tubes, making space for more cells to simultaneously and efficiently consume the oil.

The researchers observed the stretching mechanism at the interface by focusing on the dynamics of tube formation. As cell division at the interface occurs, the cells, which appear as small rods under the microscope, become tighly packed. In certain regions the cells exhibit a configuration resembling the petal arrangement found in a wild chrysanthemum. This arrangement, known as a topological defect in liquid crystal physics, begins to buldge outward from the droplet, deforming the interface and developing into a tube. The research group also succeeded in predicting both the dynamics of biofilm formation and its qualitative shape using a theoretical physical model.

Furthermore, the team demonstrated that large concentrations of dispersants, which are used during oil-spill cleanup, can have an adverse effect on A. borkumensis biofilms but further research is needed to clarify actual effects in the environment.

Therefore, this work provides insights into ways to leverage bacterial communities for enhanced environmental remediation of oceanic oil spills. The researchers believe that the understanding of biofilm formation and bacterial cooperation can help capitalize on nature's efficient methods for improving the effectiveness of current oil-spill cleanup processes.

The research leading to these results was supported by KAKENHI by the Japan Society for the Promotion of Science (JSPS) JSPS-ANR PHC SAKURA 2018 (No. 38559WJ), JSPS BRIDGE (No. BR200204), JST-ERATO (No. JPMJER1502), and by JSPS KAKENHI (No. 21H01720).

Original Paper

Title of original paper:
Alcanivorax borkumensis Biofilms Enhance Oil Degradation By Interfacial Tubulation


Associate Professor Andrew S. Utada
Institute of Life and Environmental Sciences, University of Tsukuba

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