演者： Dr. Masahiko Watanabe (渡邉 雅彦先生)
Department of Anatomy, Faculty of Medicine, Hokkaido University（北海道大学大学院医学研究科）
演題:"Glyoxal, an innovative fixative for neuroscience research"
要旨: Synaptic molecules are highly organized to fulfill neural functions. Through my neuroanatomical research on molecular organization and mechanism of glutamatergic synaptic wiring, I realize that widely-used formalin fixation often hinders synaptic molecules from histochemical detection. This is particularly true for PSD and synaptic adhesion molecules. To solve this problem, I have so far tested and adopted various antigen-retrieval and -exposing techniques, such as protease pretreatment and postembedding methods. Recently, glyoxal fixation was reported to be a better alternative for formalin fixation (Richter et al. EMBO J 37:139-159, 2018). In this seminar, I will introduce the usefulness and effectiveness of the new fixative in synaptic molecule detection.
